By Henrik Nielsen (auth.), Henrik Nielsen (eds.)
Recent perception into the transcripts generated from the mammalian genome (i.e. the transcriptome) has published that transcription is a much more complicated phenomenon than formerly concept. In RNA: equipment and Protocols, professional researchers give you the techniques and strategies used to explain the constitution of messenger RNAs and non-coding RNAs which are transcribed through RNA polymerase II because the instant gene items in mammalian cells. thinking about the constitution of the RNA items of “gene X” and the mapping of proteins linked to those RNAs, the quantity offers applicable details for non-specialists in RNA biology. Written within the hugely winning tools in Molecular Biology™ sequence structure, many chapters comprise introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, without problems reproducible laboratory protocols, and key pointers on troubleshooting and heading off recognized pitfalls. complete and useful, RNA: tools and Protocols perspectives the transcriptional panorama with an appreciation for the position that proteins play within the processing and interpretation of genetic info in an try and extra our an important wisdom of the numerous items and complex regulatory networks that outcome from it.
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