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By H. P. Zingsheim, H. Plattner (auth.), Edward D. Korn (eds.)

The contributions of electron microscopy to membrane biology were critical and, whilst, disappointing. Membranes have been recognized to exist prior to the arrival of electron microscopy and normal rules in their composition and molecular association were deduced from permeability and electric conductivity measurements, polarized gentle microscopy, and X-ray diffraction. nevertheless, the complexities of the numerous intracellular membranes and membranous organelles have been fairly now not suspected till they have been saw through the electron microscopist. One then had extra hopes that the excessive answer of the electron microscope (theoretically it may possibly unravel atomic distances) could let the visualization of the molecular structure of membranes and lead on to an less than­ status of constitution and serve as on the molecular point. This expectation has been principally unrealized. as a result nice problems encountered within the guidance of organic fabric, due to the uncertainty of the chemistry of "staining," and thanks to a number of electron optical artifacts, it's been an enormous problem simply to rationalize the saw pictures by way of the identified proof, not to mention to make use of the photographs to extend our wisdom of the molecular constitution of organic membranes. the numerous alterations between membranes with admire to operate and composition are misplaced within the common trilamellar photograph. maybe the only significant exception to this, and the key structural contribution of electron microscopy on the molecular point, has come from freeze-etch electron microscopy.

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